Stat5 is activated in response to a wide variety of ligands including IL-2, GM-CSF, growth hormone and prolactin.
Phosphorylation on residue Tyr694 is obligatory for Stat5 activation (1,2).
Stat5 has been found to be constitutively active in some leukemic cell types (3).
Phosphorylated Stat5 is found in some endothelial cells treated with IL-3, which suggests its involvement in angiogenesis and cell motility (4).
Stat5alpha and-beta are independently regulated and activated in various cell types.
For instance, both isoforms are activated in response to IFN-alpha in B cells, but only Stat5alpha is phosphorylated in response to IFN-alpha in HeLa cells (5).
Specificity/Sensitivity: Phospho-Stat5 (Tyr694) Antibody detects endogenous levels of Stat5 only when phosphorylated at Tyr694.
Applications:Suitable for use in ELISA, Western Blot and Flow Cytometry.
Other applications not tested.
Recommended Dilution:Flow Cytometry: 1:50Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Specificity：Detects endogenous levels of mouse Stat5 only when phosphorylated at Tyr694. Does not crossreact with the corresponding phospho-tyrosine residues of other Stat proteins. Species Crossreactivity: human, mouse
Isotype：IgG
Calc Applications Abbrev：E FC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic phosphopeptide corresponding to residues around Tyr694 of mouse Stat5 (KLH). Species sequence homology: bovine and rat
