Spectrin is a universally expressed membrane-associated cytoskeletal protein consisting of alpha-and beta-subunits.
This protein is important for maintaining normal membrane structure and supporting cell surface protein function.
Alpha-spectrin is one of the primary targets cleaved by caspases during apoptosis.
The full length 240kD protein can be cleaved at several sites within its sequence by activated caspases, to yield Nterminal 150kD, C-terminal 120kD and 35kD major products.
Cleavage of alpha-fodrin leads to membrane malfunction and cell shrinkage.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Recognizes endogenous levels of the large fragment of human Spectrin, alpha resulting from cleavage at aspartic acid 1185. Does not recognize full length Spectrin, alpha.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to amino-terminal residues surronding Asp1185 from human Spectrin, alpha.
