Fodrin (also named nonerythroid spectrin) is a universally expressed membrane-associated cytoskeletal protein consisting of alpha-and beta-subunits (1).
This protein is important for maintaining normal membrane structure and supporting cell surface protein function (1).
Alpha-fodrin is one of the primary targets cleaved by caspases during apoptosis.
The full length 240kD protein can be cleaved at several sites within its sequence by activated caspases, to yield Nterminal 150kD, C-terminal 120kD and 35kD major products (2–5).
Cleavage of alpha-fodrin leads to membrane malfunction and cell shrinkage.
Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000, incubate membrane with diluted antibody in 5% nonfat dry milk, TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Specificity：Recognizes total levels of human endogenous full length alphafodrin (240kD) and its large fragment (150kD) following cleavage by caspase(s) at Asp1185.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to residues surrounding Asp1185 of human alpha-fodrin.
