84-1037-46　SAPK, JNK (Stress-Activated Protein Kinase, Jun-terminal Kinase) 200ul　S0096-04E

特徴

• The stress-activated protein kinase/Jun terminal kinase SAPK/JNK is potently and preferentially activated by a variety of environmental stresses, including UV and gamma radiation, ceramides, inflammatory cytokines and, in some instances, by growth factors and GPCR agonists.
• As with the other MAPKs, the core signaling unit is composed of an MAPKKK, typically MEKK1–4, or a member of the mixed lineage kinases (MLKs) that would phosphorylate and activate MKK4–7, the SAPK/JNK kinase.
• Stress signals are delivered to this cascade by members of small GTPases of the Rho family (Rac, Rho, cdc42).
• Both Rac1 and cdc42 mediate the stimulation of MEKKs and MLKs.
• Alternatively, MKK4–7 can be activated in a pathway independent of small GTPases via stimulation of a member of the germinal center kinase (GCK) family.
• There are three SAPK/JNK genes with further diversification from alternative splicing.
• SAPK/JNK, when active as a dimer, can translocate to the nucleus where it regulates transcription through its effects on c-Jun, ATF-2 and other transcription factors.
• Applications:Suitable for use in Western Blot.
• Other applications have not been tested.
• Recommended Dilution:Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage, store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.