90 kD ribosomal S6 kinases (RSK1-3) are a family of broadly expressed serine/ threonine kinases activated by extracellular signal-regulated protein kinases (Erk1 and 2) in response to many growth factors, polypeptide hormones and neurotransmitters (1).
p90RSK is activated by MAPK in vitro and in vivo via phosphorylation (4).
Several sites, such as Ser380, Thr359 and Ser363, are important for its activation (3).
RSK3 resembles p90RSK by having two kinase domains connected by a regulator linker region (2).
Phosphorylation of p90RSK (and likely RSK2 and RSK3) in the linker region is critical for its activity (3).
Applications:Suitable for use in Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:1000Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Specificity：Recognizes endogenous levels of rat p90RSK only when phosphorylated at Thr359/Ser363. Does not recognize p90RSK phosphorylated at other sites. Some crossreactivity with RSK3 when phosphorylated at the homologous sites. Does not crossreact with phospho-RSK2 or MSK1. Species Crossreactivity: human, rat, monkey and mouse.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mk Mo Rt
Immunogen：Synthetic phosphopeptide corresponding to residues surrounding Thr359/Ser363 of rat RSK1.
