84-1037-36　Raf, phosphorylated (Ser259) (Raf-1, c-Raf) 100ul　R0495-09A

特徴

• Raf-1, a mitogen-activated protein kinase, is the main effector recruited by GTP-bound Ras in order to activate the MAP kinase pathway (1).
• 14–3–3 binds to c-Raf at two phospho-serine residues: Ser259 and Ser621 (2).
• Mutation of Ser259 to alanine constitutively activates Raf kinase activity, but mutation of Ser621 to alanine results in an inactive kinase (2).
• Aktmediated phosphorylation of Ser259 has been shown in vivo to inhibit Raf kinase activity (4).
• Phosphorylation at Ser338 and Tyr341 of Raf-1 is a critical step for Raf-1 activation (5,6).
• p21-activated protein kinase (Pak) has been shown to phosphorylate Raf at Ser338 and to induce Raf-1 activity.
• Applications:Suitable for use in Western Blot and Immunoprecipitation.
• Other applications have not been tested.
• Recommended Dilution:Western Blot: 1:1000 Immunoprecipitation: 1:50Optimal dilutions to be determined by the researcher.
• Storage and Stability: May be stored at 4°C for short-term only.
• For long-term storage, store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.