Phosphorylation of the alpha subunit of eukaryotic initiation factor 2 is a well documented mechanism of downregulating protein synthesis under a variety of stress conditions.
Eukaryotic initiation factor 2 binds GTP and Met-tRNAi and transfers the Met-tRNA to the 40S subunit to form the 43S preinitiation complex.
For eIF2 to promote a new round of translation initiation, GDP must be exchanged for GTP, a reaction catalyzed by eIF2B.
Kinases activated by viral infection (PKR), endoplasmic reticulum stress (PERK/PEK), amino acid deprivation (GCN2) and hemin deficiency (HRI) can phosphorylate the alpha subunit of eIF2 (5,6).
This phosphorylation stabilizes the eIF2- GDP-eIF2B complex inhibiting the turnover of eIF2B.
Induction of PKR by IFN-gamma and TNF-alpha, or stress provoked by depletion of endoplasmic reticulum calcium levels, induces potent phosphorylation of eIF2alpha at Ser51 (7,8).
Applications:Suitable for use in Western Blot.
Other applications have not been tested.
Recommended Dilution:Western Blot: 1:1000, incubate membrane with diluted primary antibody in 5% BSA, 1X TBS, 0.1% Tween 20 at 4°C with gentle shaking, overnight Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Specificity：Detects endogenous eIF2alpha only when phosphorylated at Ser51. Does not recognize elF2alpha phosphorylated at other sites. Species Crossreactivity: Human, mouse, monkey, rat and Drosophila.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Dr Hu Mk Mo Rt
Immunogen：Synthetic phosphopeptide corresponding to residues around Ser51 of human eIF2alpha.
