Type 1 protein phosphatase (PP1), a serine/threonine phosphatase, is highly conserved in eukaryotic cells.
Four isoforms of PP1 have been characterized: PP1 alpha, PP1 delta, PP1 gamma1 and PP1 gamma2.
Involvement in the cell cycle regulation is one of the biological functions of PP1.
It has been illustrated that PP1 dephosphorylates pRB and cdc25 during mitosis.
The activity and specificity of PP1 is highly regulated by regulatory subunits and targeting proteins.
In addition, a cell-cycle dependent phosphorylation at threonine 320 of PP1• by cdc2 kinase has been reported recently, which inhibits PP1• activity.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Immunohistochemistry (Paraffin): 1:50Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Specificity：Recognizes human PP1 alpha only when phosphorylated at Thr320. May crossreact with phosphorylated PP1 isoforms delta or gamma. Species Crossreactivity: Mouse and rat.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic phosphopeptide corresponding to residues surrounding Thr320 of human PP1α.
