The protein kinase C-related kinases (PRKs) are a subfamily of serine/threonine specific kinases with a catalytic domain highly homologous to the PKC family (1–3).
They are effectors of Rho family GTPase (4–6) and activated by fatty acids and phospholipids in vitro (7,8).
Activation in vitro and in vivo involves the activation loop phosphorylation of PRK1 (Thr778)/PRK2 (Thr816) by PDK1 (9,10).
Recommended Dilution: Western Blotting: 1:1000Immunoprecipitation: 1:100Optimal dilutions to be determined by the researcher.
Storage and Stability: May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Specificity：Detects total endogenous levels of human PRK2. Does not crossreact with PRK1. Species Crossreactivity: Mouse and rat.
Isotype：IgG
Calc Applications Abbrev：E IP WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide (KLH coupled) derived from the N-terminal sequence of human PRK2.
