The protein kinase C–related kinases (PRKs) are a subfamily of serine/threonine specific kinases with a catalytic domain highly homologous to the PKC family (1–3).
They are effectors of Rho family GTPase (4–6) and activated by fatty acids and phospholipids in vitro (7,8).
Activation in vitro and in vivo involves the activation loop phosphorylation of PRK1 (Thr778)/PRK2 (Thr816) by PDK1 (9,10).
Applications:Suitable for use in ELISA, Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability: May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Specificity：Detects PRK1 or PRK2 only when phosphorylated at Thr778 or Thr816, respectively. Species Crossreactivity: Mouse and rat.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Thr778 of human PRK1.
