Activation of PKC is one of the earliest events in the cascade leading to a variety of cellular responses such as secretion, gene expression, proliferation and muscle contraction (1,2).
Protein kinase D (PKD), also called PKCmu, is a serine/threonine kinase (3,4).
Activation of PKD/PKCmu is dependent on the phosphorylation of two activation loop sites, namely serine 744 and serine 748, via a PKC-dependent signal transduction pathway (5).
In addition to the two activation loop sites, the C-terminal serine 916 has been identified as an autophosphorylation site for PKD/ PKCmu.
Phosphorylation at the Ser916 site correlates with PKD/PKCmu catalytic activity (6).
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Specificity：Recognizes mouse PKD/PKCmu (MW 115kD) only when phosphorylated at Ser744/748 at 115kD. Does not crossreact with PKD/PKCmu phosphorylated at other sites, nonphosphorylated PKD/PKCmu or other PKC isoforms. May crossreact with isoforms PKD2 and PKD3/PKC in some species. Species Crossreactivity: human, monkey and rat.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mk Mo Rt
Immunogen：Synthetic phosphopeptide corresponding to residues surrounding Ser744/748 of mouse PKD (KLH).
