Presenilin 1 and presenilin 2 are transmembrane proteins belonging to the presenilin family.
Mutation of presenilin genes has been linked to early onset of Alzheimer’s disease, probably functioning through its associated gamma-secretase activity for amyloid-beta protein processing (1,2).
Endogenous presenilin mainly exists in a heterodimeric complex formed from the endoproteolytically processed N-terminal (34kD) and C-terminal (~20kD) fragments (2,3).
Applications:Suitable for use in Western Blot and Immunoprecipitation.
Other applications have not been tested.
Recommended Dilutions:Western Blot: 1:1000 Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher.
Positive Control:293 and HeLa (human), NIH/3T3 and L929 (mouse), and C6 and PC12 (rat) cell lines
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and immunoaffinity chromatography.
Form：Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Specificity：Recognizes endogenous levels of the carboxy-terminal fragment of human presenilin 2 (~20kD) and the full length protein (54kD) to a lesser extent. Does not crossreact with endogenous levels of presenilin 1. Species Crossreactivity: monkey, mouse, rat.
Isotype：IgG
Calc Applications Abbrev：IP WB
Calc Crossreactivity：Hu Mk Mo Rt
Immunogen：Synthetic peptide corresponding to residues surrounding 330aa of human presenilin 2.
