Phosphoinositide-specific phospholipase C (PLC) plays a significant role in transmembrane signaling.
In response to extracellular stimuli, such as hormones, growth factors and neurotransmitters, PLC hydrolyzes phosphatidylinositol 4,5-biphosphate (PIP2) to generate two secondary messengers: inositol 1,4,5- triphosphate (IP3) and diacylglycerol (DAG) (1).
At least four families of PLCs have been identified: PLCbeta, PLCgamma, PLCdelta and PLCepsilon.
The PLCbeta subfamily includes four members, PLCbeta1–4.
All four members of the subfamily are activated by alpha-or beta-gamma-subunits of the heterotrimeric G-proteins (2,3).
Phosphorylation is one of the key mechanisms that regulates the activity of PLC.
Phosphorylation of serine 1105 by PKA or PKC inhibits PLCbeta3 activity (4,5).
Serine 537 of PLCbeta3 is phosphorylated by CaMKII and this phosphorylation may contribute to the basal activity of PLCbeta3.
PLCgamma is activated by both receptor and nonreceptor tyrosine kinases (6).
PLCgamma forms a complex with EGF and PDGF receptors, which leads to the phosphorylation of PLCgamma1 on tyrosine residues 771, 783 and 1245 (7).
Phosphorylation by Syk at tyrosine 783 activates the enzymatic activity of PLCgamma1 (8).
Applications:Suitable for use in ELISA, Western Blot, Immunofluorescence and Flow Cytometry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000 incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight
Flow Cytometry: 1:25 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Specificity：Recognizes human PLC gamma 1 only when activated by phosphorylation at Tyr783. It does not crossreact with phosphorylated PLC gamma 2 or other PLCs. Species Crossreactivity: Mouse and rat.
Isotype：IgG
Calc Applications Abbrev：E IHC IP WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic phosphopeptide corresponding to residues surrounding Tyr783 of human PLC gamma 1.
