Phosphoinositide-specific phospholipase C (PLC) plays a significant role in transmembrane signaling.
In response to extracellular stimuli, such as hormones, growth factors and neurotransmitters, PLC hydrolyzes phosphatidylinositol 4,5-biphosphate (PIP2) to generate two secondary messengers: inositol 1,4,5- triphosphate (IP3) and diacylglycerol (DAG) (1).
At least four families of PLC5, PLCbeta, PLCgamma, PLCdelta and PLCepsilon have been identified so far.
The PLCbeta subfamily includes four members, PLCbeta1–4.
All four members of the subfamily are activated by alpha-or beta-gamma-subunits of the heterotrimeric G-proteins (2,3).
Phosphorylation is one of the key mechanisms that regulates the activity of PLC.
Phosphorylation of serine 1105 by PKA or PKC inhibits PLCbeta3 activity (4,5).
Serine 537 of PLCbeta3 is phosphorylated by CaMKII and this phosphorylation may contribute to the basal activity of PLCbeta3.
PLCgamma is activated by both receptor and nonreceptor tyrosine kinases (6).
PLCgamma forms a complex with EGF and PDGF receptors, which leads to the phosphorylation of PLCgamma1 on tyrosine residues 771, 783 and 1245 (7).
Phosphorylation by Syk at tyrosine 783 activates the enzymatic activity of PLCgamma1 (8).
Applications:Suitable for use in Western Blot and Immunoprecipitation.
Other applications have not been tested.
Recommended Dilution:Western Blot: 1:1000Immunoprecipitation: 1:50Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Specificity：Recognizes endogenous levels of total human PLC gamma1 protein at ~155kD. Does not crossreact with PLCgamma2. Species Crossreactivity: mouse and rat.
Isotype：IgG
Calc Applications Abbrev：IP WB
Calc Crossreactivity：Hu Mk Rt
Immunogen：Synthetic peptide corresponding to the C-terminal residues of human Phospholipase C, gamma 1 (PLCg1).
