Phosphoinositide-specific phospholipase C (PLC) plays a significant role in transmembrane signaling.
In response to extracellular stimuli, such as hormones, growth factors and neurotransmitters, PLC hydrolyzes phosphatidylinositol 4,5-biphosphate (PIP2) to generate two secondary messengers: inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG) (1).
At least four families of PLC5, PLCbeta, PLCgamma, PLCdelta and PLCepsilon have been identified so far.
The PLCbeta subfamily includes four members, PLCbeta1-4.
All four members of the subfamily are activated by alpha-or betagamma- subunits of the heterotrimeric G-proteins (2,3).
Phosphorylation is one of the key mechanisms that regulates the activity of PLC.
Phosphorylation of serine 1105 by PKA or PKC inhibits PLCbeta3 activity (4,5).
Serine 537 of PLCbeta3 is phosphorylated by CaMKII and this phosphorylation may contribute to the basal activity of PLCbeta3.
PLCgamma is activated by both receptor and nonreceptor tyrosine kinases (6).
PLCgamma forms a complex with EGF and PDGF receptors, which leads to the phosphorylation of PLCgamma1 on tyrosine residues 771, 783 and 1245 (7).
Phosphorylation by Syk at tyrosine 783 activates the enzymatic activity of PLCgamma1 (8).
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000.
Incubate membrane with diluted antibody in TBS, 5% BSA, 0.1% Tween-20 at 4°C with gentle shaking, overnight
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Specificity：Recognizes endogenous levels of human PLCbeta3 only when phosphorylated at Ser1105. Does not crossreact with phosphorylated PLCbeta1, PLCbeta2, PLCbeta4 or other PLCs. Species Crossreactivity: rat and monkey.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mk Mo Rt
Immunogen：Synthetic phosphopeptide corresponding to residues surrounding Ser1105 of human PLCbeta3.
