84-1036-84　NOS III, phosphorylated (Ser1177) (Endothelial Nitric Oxide Synthase, eNOS, NOS 3) 100ul　N5350-26

特徴

• Endothelial nitric-oxide synthase (eNOS) is an important enzyme in the cardiovascular system.
• It catalyzes the production of nitric oxide (NO), a key regulator of blood pressure, vascular remodeling and angiogenesis (1,2).
• eNOS is regulated by phosphorylation at multiple sites.
• The two most throughly studied sites are the activation site serine 1177 and the inhibitory site threonine 495 (3).
• Several protein kinases including Akt/PKB, PKA and AMPK activate eNOS by phosphorylating Ser1177 in response to various stimuli (4,5).
• In contrast, bradykinin and hydrogen peroxide activate eNOS activity by promoting Thr495 dephosphorylation (6,7).
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.