The transcriptional nuclear factor kappaB (NF-kappaB) plays a pivotal role in inflammatory and immune responses (1,2).
NF-kappaB is composed of a heterodimer of p65 and p50 subunits in most cell types and is sequestered in the cytoplasm by its inhibitory proteins, the IkappaBs (3-5).
NF-kappaB-activating agents can induce the phosphorylation of IkappaBs; the phosphorylation targets them for rapid degradation through a ubiquitin-proteasome pathway, thereby releasing NF-kappaB to enter the nucleus for gene expression (6-8).
It has been shown that the p65 NF-kappaB subunit is also phosphorylated during the phosphorylation and degradation of IkappaBs; the phosphorylation site is Ser536 in the C-terminal transactivation domain (9).
Applications:Suitable for use in ELISA and Western Blot.
Others not tested.
Recommended Dilution:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Specificity：Detects human NF-kappaB p65 only when phosphorylated at Ser536 at 75kD. It does not crossreact with the p50 subunit or other related proteins. Species Crossreactivity: Mouse, monkey and rat
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mk Mo Rt
Immunogen：Synthetic phosphopeptide corresponding to residues surrounding Ser536 of human NF-kappaB p65 (KLH). Species sequence homology: canine and porcine
