Eukaryotic initiation factor 4E (eIF4E) binds to the mRNA cap structure thereby mediating the initiation of translation.
eIF4E interacts with eIF4G, which serves as a scaffold protein for the assembly of eIF4E and eIF4A to form the eIF4F complex.
eIF4B is thought to assist the eIF4F complex in translation initiation.
Upon activation by mitogenic and/or stress stimuli mediated by Erk and p38 MAPK, Mnk1 has been shown to phosphorylate eIF4E at Ser209 in vivo.
Two Erk and p38 MAPK phosphorylation sites have been identified in mouse Mnk1, Thr197 and Thr202, which are essential for Mnk1 kinase activity.
The C-terminal region of eIF4G also contains serum-stimulated phosphorylation sites, including Ser1108, Ser1148 and Ser1192.
It is known that their phosphorylation is blocked by the PI3 kinase inhibitor LY294002 and by the FRAP/mTOR inhibitor rapamycin.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Specificity：Recognizes endogenous levels of mouse Mnk1 at ~50kD only when phosphorylated at Thr198/203 (Thr197/Thr202 in human). Crossreacts with phosphorylated Mnk2a and Mnk2b. Species Crossreactivity: rat, human and monkey.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mk Mo Rt
Immunogen：Synthetic phosphopeptide corresponding to residues surrounding Thr198 and Thr203 of mouse Mnk1 (Thr197/Thr202 in human).
