MKK3 and MKK6 are two closely related dual-specificity protein kinases that activate p38 MAP kinase (1-5).
MKK3 and MKK6 both phosphorylate and activate p38 MAP kinase at its activation site, Thr-Gly-Tyr.
They do not phosphorylate or activate the two other MAP kinases, Erk1/2 or SAPK/JNK.
Phosphorylation of p38 MAP kinase dramatically stimulates the ability of p38 MAP kinase to phosphorylate protein substrates such as ATF-2 and Elk-1.
MKK3 and MKK6 are both activated by different forms of cellular stress and inflammatory cytokines (4,5).
Activation of MKK3 and MKK6 occurs through phosphorylation of serine and threonine residues at sites Ser189 and Thr193 for MKK3 (2), and Ser207 and Thr211 for MKK6 (4,5).
Applications:Suitable for use in ELISA, Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Immunoprecipitation: 1:250Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added. Also available without BSA & glycerol. See M2363-15C.
Specificity：Recongizes endogenous levels of human MKK3/MKK6 only when phosphorylated at Ser189/207. Does not recognize the corresponding phosphorylated residues of MEK1, MEK2 or MKK4/SEK1. Species Crossreactivity: Mouse and rat.
Isotype：IgG
Calc Applications Abbrev：IP WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic phosphopeptide corresponding to residues around Ser189/207 of human MKK3.
