Both p44 and p42 MAP kinases (Erk1 and Erk2) function in a protein kinase cascade that plays a critical role in the regulation of cell growth and differentiation (1-4).
MAP kinases are activated by a wide variety of extracellular signals including growth and neurotrophic factors, cytokines, hormones and neurotransmitters.
Activation of MAP kinases occurs through phosphorylation of threonine and tyrosine (202 and 204 of human MAP kinase [Erk1] or 183 and 185 of rat Erk2) at the sequence T*EY* by a single upstream MAP kinase kinase (MEK) (5,6).
Both kinases are known to weakly autophosphorylate on tyrosine (5).
Applications:Suitable for use in Flow Cytometry, ELISA, Western Blot, Immunoprecipitation, Immunofluorescence and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000.
Incubate membrane with diluted antibody in TBS, 5% BSA, 0.1% Tween-20 at 4°C with gentle shaking, overnight
Immunoprecipitation: 1:50Immunofluorescence (IF-IC): 1:1000Flow Cytometry: 1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservatives added.
Specificity：Detects endogenous levels of human p42 and p44 MAP kinase (Erk1 and Erk2) only when phosphorylated either individually or dually at Thr202 and Thr204 of Erk1 (Thr185 an Tyr187 of Erk2). Does not crossreact with the corresponding phosphorylated residues of either JNK/SAPK or p38 MAP kinase, or non-phosphorylated Erk1/2. Species Crossreactivity: chicken, hamster, mouse, rat, zebrafish. bovine and monkey.
Isotype：IgG
Calc Applications Abbrev：E FC IC IF IP WB
Calc Crossreactivity：Bo Ch Hm Hu Mk Mo Rt
Immunogen：Synthetic phosphopeptide corresponding to residues around Thr202/Tyr204 of human p44 MAP kinase (KLH).
