P38 is the newest member of the MAP kinase family.
It is a reactivating kinase.
It is activated in response to inflammatory cytokines, endotoxins, and osmotic stress.
p38 MAP kinase (MAPK), also called RK or CSBP, is the mammalian homologue of the yeast HOG kinase and participates in a signaling cascade controlling cellular responses to cytokines and stress.
Four isoforms of p38 MAP kinase, alpha, beta, gamma and delta, have been identified so far.
Like the SAPK/JNK pathway, p38 MAP kinase is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharides (LPS), UV light and growth factors.
MKK3 and SEK activate p38 MAP kinase by phosphorylation at threonine 180 and tyrosine 182.
Activated p38 MAP kinase has been shown to phosphorylate and activate MAPKAP kinase 2 and to phosphorylate the transcription factors ATF-2, Max and MEF2.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry and Flow Cytometry.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:1000.
Incubate membranes with diluted antibody, 1X TBS, 5% BSA, 0.1% Tween-20 at 4ºC with gentle shaking overnight.
Immunohistochemistry (Paraffin): 1:100.
Antigen retrieval with citrate buffer required
Immunofluorescence (IC): 1:50Flow Cytometry: 1:400Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography
Form：Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Recognizes endogenous levels of total human p38 alpha, beta, or gamma MAP kinase protein (40kD). Does not recognize either p38 delta JNK/SAPK or p44/42 MAP kinase. Species Crossreactivity: mouse, monkey, rat and guinea pig
Isotype：IgG
Calc Applications Abbrev：FC IC IF IHC WB
Calc Crossreactivity：Gp Hu Mk Mo Rt
Immunogen：Synthetic peptide corresponding to sequence of human p38 MAPK. Species Sequence Homology: chicken
