84-1036-63　Lyn, phosphorylated (Tyr507) (p53/56lyn) 100ul　L8050-11

特徴

• Lyn is a member of the src family of tyrosine kinases responsible for the regulation of cell signaling mechanisms.
• Lyn’s function has been studied in cells of hematopoetic origin where it is associated with the high affinity IgE receptor in basophils and with the p120 Ras-GAP in thrombin-stimulated platelets.
• Lyn, one of the Src family members, is predominantly expressed in hematopoietic cells (1).
• Two tyrosyl residues have been reported to play a crucial role in the regulation of protein tyrosine kinases of the Src family: autophosphorylation of tyrosine 396 (equivalent to tyrosine 416 of c-Src) located in the catalytic domain correlates with enzyme activation, while Csk-mediated phosphorylation of the C-terminal tyrosine 507 (equivalent to tyrosine 527 of c-Src) inactives the kinases.
• Lyn was tyrosine phosphorylated and activated upon association with cell surface receptors such as the B cell Ag recepter (BCR) and CD40 (2–4).
• Knockout mice have shown that the net effect of Lyn deficiency is to render B cells hyperactive to BCR stimulation (5–7), suggesting that the most critical role for Lyn in vivo is in the downmodulation of B cell responses.
• Lyn is also involved in controlling the migration and development of specific B cell populations (8).
• Applications:Suitable for use in Western Blot.
• Other applications have not been tested.
• Recommended Dilution:Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnightOptimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.