Lck, a member of the Src family of protein tyrosine kinases, is essential for T-lymphocyte activation and differentiation (1,2).
It is associated with a variety of cell surface receptors and plays a key role in T-cell antigen receptor linked signal transduction pathways.
The activity of Lck is regulated by protein kinases and phosphatases.
Phosphorylation of the C-terminal tyrosine 505 serves to downregulate Lck catalytic activity, while phosphorylation at tyrosine 394 leads to an increase in Lck activity (3,4).
The protein tyrosine phosphatase SHP-1 has been shown to dephosphorylate Lck at tyrosine 394 (5).
Applications:Suitable for use in Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Immunoprecipitation: 1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol
Specificity：Recognizes endogenous levels human Lck only when phosphorylated at Tyr505. Can crossreact with phospho-Lyn (Tyr507) due to high sequence homology. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：E IP WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic phosphopeptide corresponding to residues surrounding Tyr505 of human Lck.
