Lck, a member of the Src family of protein tyrosine kinases, is essential for T-lymphocyte activation and differentiation.
It is associated with a variety of cell surface receptors and plays a key role in T-cell antigen receptor linked signal transduction pathways.
The activity of Lck is regulated by protein kinases and phosphatases.
Phosphorylation of the C-terminal tyrosine 505 serves to downregulate Lck catalytic activity, while phosphorylation at tyrosine 394 leads to an increase in Lck activity.
The protein tyrosine phosphatase SHP-1 has been shown to dephosphorylate Lck at tyrosine 394.
Applications:Suitable for use in ELISA, Western Blot, Immunoprecipitation and Flow Cytometry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Immunoprecipitation: 1:50Flow Cytometry: 1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Specificity：Detects total (phosphorylation-state independent) Lck protein. Detects endogenous levels of Lck. Species Crossreactivity: human and mouse.
Isotype：IgG
Calc Applications Abbrev：E FC IP WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to residues surrounding the C-terminus of human Lck.
