Lamins are nuclear membrane structural components that are important in maintaining normal cell functions such as cell cycle control, DNA replication and chromatin organization (1–3).
Lamin A is cleaved by caspase-6 and serves as a marker for caspase- 6 activation.
During apoptosis, the 70kD lamin A is specifically cleaved to a large (40–45kD) and a small (28kD) fragment (3,4).
The cleavage of lamins results in nuclear disregulation and cell death (5,6).
Nuclear lamins form a network of filaments at the nucleoplasmic site of the nuclear membrane.
Two main subtypes of nuclear lamins can be distinguished: A-type lamins and B-type lamins.
The A-type lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e.
lamin A, lamin C and lamin Adel 10, while the B-type lamins include two proteins arising from two distinct genes, i.e.
lamin B1 and lamin B2.
Type A lamins comprise a set of three proteins arising from the same gene by alternative splicing: lamins A, C, and Adel 10.
B-type lamins include two proteins arising from two distinct genes, i.e.
lamin B1 and lamin B2.
Mutations in A-lamins give rise to a range of genetic disorders, including autosomal dominant Emery-Dreifuss muscular dystrophy (AD-EDMD), dilated cardiomyopathy and Dunnigan-type familial partial lipodystrophy.
Aberrant expression patterns of nuclear lamins have been described in various types of cancer depending on the subtype of cancer, its aggressiveness, proliferative capacity and degree of differentiation.
In general, the expression of A-type lamins (lamins A and C) has been correlated with a non-proliferating, differentiated state of cells and tissues.
Lamins A and C, the products of the LMNA gene, are nuclear intermediate filament proteins and are the major structural components of the lamina network that underlies and supports the nuclear envelope.
Expression of A-lamin coincides with cell differentiation and may be related protein’s abnormal expression and organization in neoplastic tissues and cells.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Specificity：Detects the small fragment of lamin A (and lamin C) resulting from cleavage at Asp230 by caspase-6. Does not crossreact with full length lamin A or lamin C. Species Crossreactivity: Mouse and rat.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to C-terminal residues adjacent to Asp230 in human lamin A.
