Human Skb1Hs methyltransferase (also called JBP1), a homologue of yeast protein Skb1 and Hsl7p (1,2), is composed of 637 amino acid residues and contains motifs conserved among protein methyltransferases.
It methylates histones and MBP in vitro (2).
Yeast Hsl7p is involved in regulation of cell cycle progression through G2 by negatively regulating Swe1p, a protein tyrosine kinase that phosphorylates and inhibits Cdc28p (3).
An Hsl7p homologue, Skb1, was identified in fission yeast by virtue of its yeast twohybrid interaction with Shk1p, a p21 (cdc42p/Rac) activated kinase (PAK) (4).
Both proteins belong to the protein methyltransferase superfamily (5).
Interestingly, human Skb1Hs methyltransferase was shown to interact with Jak kinases.
This suggests the possibility that the Skb1Hs methyltransferase could link Jak to a PAK signaling pathway in mammalian cells.
Applications:Suitable for use in Western Blot and Immunoprecipitation.
Other applications have not been tested.
Recommended Dilution:Western Blot: 1:1000Immunoprecipitation: 1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA and 50% glycerol. No preservative added.
Specificity：Recognizes endogenous levels of total human Skb1Hs methyltransferase protein at 70kD. Does not crossreact with other related mammalian methyltransferases. Species Crossreactivity: monkey, mouse and rat.
Isotype：IgG
Calc Applications Abbrev：IP WB
Calc Crossreactivity：Hu Mk Mo Rt
Immunogen：Synthetic peptide corresponding to residues surrounding 100aa of human JBP1 Methyltransferase (KLH). MW: 70kD.
