84-1036-53　IRS-1 (Insulin Receptor Substrate 1) 100ul　I8700-01

特徴

• Insulin Receptor Substrate (IRS-1) is one of the major substrates of Insulin Receptor Kinase.
• IRS-1 contains multiple tyrosine phosphorylation motifs that serve as docking sites for SH2 domain-containing proteins (Grb2, p85, SHP-2, Fyn and others) and which mediate the metabolic and growth promoting functions of insulin.
• IRS-1 also contains over 30 potential Ser/Thr phosphorylation sites for kinases, such as PKA, PKC and MAPK.
• The phosphorylation of Ser 612 and Ser636/639 are mediated by PKA and mTOR pathways, respectively, resulting in an inhibition of insulin signaling in the cell and suggesting a potential mechanism for insulin resistance in some models of obesity.
• Once phosphorylated by insulin receptor, IRS-1 recruits the downstream components that mediate the insulin signaling.
• IRS-1 protein contains a conserved pleckstrin homology (PH) domain located at its amino terminus, adjacent to a phospho-tyrosine binding (PTB) domain.
• Applications:Suitable for use in ELISA, Western Blot and Immunoprecipitation.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 1:1000Immunoprecipitation: 1:50Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.