Type I insulin-like growth factor receptor (IGF1R, IGF-IR) is a transmembrane tyrosine kinase.
It is widely expressed among many cell types in fetal and postnatal tissues and many cell lines.
Upon binding of its ligands, IGF-I and IGF-II, autophosphorylation of receptors occurs.
The triple tyrosine cluster (Tyr1131, Tyr1135 and Tyr1136) within the kinase domain is the earliest major site of autophosphorylation.
Phosphorylation of these three tyrosine residues is necessary for activation of kinases.
Insulin receptors (IRs) share significant similarity with IGF-I receptors in structure and function.
There is also an equivalent triple tyrosine cluster (Tyr1146, Tyr1150 and Tyr1151) in its activation loop of the kinase domain.
Tyrosine autophosphorylation of the receptors is one of the earliest cellular responses to insulin stimulation.
The autophosphorylation begins by phosphorylation of Tyr1146 and either Tyr1150 or Tyr1151.
The full activation of receptor kinase activity depends on the triple tyrosine phosphorylation.
Applications:Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry.
Other applications have not been tested.
Recommended Dilution:Western Blot: 1:1000Immunoprecipitation: 1:50Immunocytochemistry (ABC): 1:50Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Specificity：Detects endogenous levels of Tyr1131 phosphorylated IGF-I receptor and Tyr1146 phosphorylated insulin receptor. Slightly crossreactivity with activated PDGF, FGF and MET receptors. Does not crossreact with other tyrosine-phosphorylated proteins. Species Crossreactivity: Mouse and rat.
Isotype：IgG
Calc Applications Abbrev：E IC IP WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic phosphopeptide corresponding to residues of human IGF-I Receptor β.
