Modulation of the chromatin structure plays an important role in the regulation of transcription in eukaryotes.
The nucleosome, made up of four core histone proteins (H2A, H2B, H3 and H4), is the primary building block of chromatin (1).
The N-terminal tail of core histones undergoes different post-translational modifications including acetylation, phosphorylation and methylation (2-4).
These modifications occur inresponse to cell signal stimuli and have a direct effect on gene expression.
In most species, the histone H2B is primarily acetylated at lysines 5, 12, 15 and 20 (4,5).
Histone H3 is primarily acetylated at lysines 9, 14, 18 and 23 (2,3).
Acetylation at lysine 9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3).
Phosphorylation at Ser10 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (6).
Applications:Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunofluorescence.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:1000Immunoprecipitation: 1:25Immunohistochemistry (Paraffin): 1:25Immunofluorescence (IF-IC): 1:3200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Specificity：Recognizes endogenous levels of Lys8 acetylated histone H4. It does not cross-react with other acetylated histones. Species Crossreactivity: monkey, mouse and rat.
Isotype：IgG
Calc Applications Abbrev：E FC IC IHC IP WB
Calc Crossreactivity：Hu Mk Mo Rt
Immunogen：Synthetic acetylpeptide corresponding to residues surrounding Lys8 of human histone H4.
