Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes.
The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3 and H4), is the primary building block of chromatin (1).
The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation and ubiquitination (2-5).
These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, on gene expression (6).
In most species, histone H2B is primarily acetylated at Lys5, 12, 15 and 20 (4, 7).
Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27 and 56.
Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2, 3).
Phosphorylation at Ser10, Ser28 and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10).
Phosphorylation of Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin.
Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation of H3 Thr3 in prophase and its dephosphorylation during anaphase (11).
Applications:Suitable for use in ELISA, Western Blot and Immunofluorescence.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:1000Immunofluorescence (IF-IC): 1:1600Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Recognizes endogenous levels of human histone H4 acetylated at Lys12 at ~11kD. Does not cross-react with other acetylated histones. Species Crossreactivity: monkey, mouse and rat.
Isotype：IgG
Calc Applications Abbrev：E IF WB
Calc Crossreactivity：Hu Mk Mo Rt
Immunogen：Synthetic acetylated peptide corresponding to residues surrounding Lys12 of human histone H4.
