Modulation of the chromatin structure plays an important role in the regulation of transcription in eukaryotes.
The nucleosome, made up of four core histone proteins (H2A, H2B, H3 and H4), is the primary building block of chromatin (1).
The N-terminal tail of core histones undergoes different post-translational modifications including acetylation, phosphorylation and methylation (2–4).
These modifications occur in response to cell signal stimuli and have a direct effect on gene expression.
In most species, the histone H2B is primarily acetylated at lysines 5, 12, 15 and 20 (4,5).
Histone H3 is primarily acetylated at lysines 9, 14, 18 and 23 (2,3).
Acetylation at lysine 9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3).
Phosphorylation at Ser10 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (6)
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography and immunoaffinity chromatography.
Form：Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Specificity：Recognizes human histone H3 only when methylated at Arg2 at 17kD. Does not crossreact with other histone members.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic methyl-Arg2 peptide corresponding to residues surrounding Arg2 of human histone H3 (KLH).
