84-1036-37　Histone Deacetylase 3 (HDAC 3) 100ul　H5109-48

特徴

• The histone tail acetylation promotes transcriptional activation.
• The identification of histone acetyltransferases (HATs) and their large multiprotein complexes has yielded important insights into how these enzymes regulate transcription (1,2).
• HAT complexes interact with sequence-specific activator proteins and target specific genes.
• In addition to histones, some HATs can acetylate nonhistone proteins, suggesting multiple roles for these enzymes (3).
• In another respect, histone deacetylation is involved in silencing of gene transcription (4).
• Mammalian histone deacetylases can be divided into two classes on the basis of their similarity to various yeast histone deacetylases (5).
• The first class is represented by its closeness to the yeast Rpd3- like proteins (HDAC 1, 2, 3 and 8), and the second class, which has similarities to yeast Hda1-like proteins (HDAC 4, 5, 6 and 7).
• Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000Immunohistochemistry: 1:50 (Paraffin)Immunocytochemistry: 1:50 (ABC detection method)
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.