84-1036-35　His-Tag (6-Histidine) 100ul　H4951

特徴

• A variety of plasmids contain DNA that encode an N-terminal tag consisting of six histidine (His) residues, followed by an extended multiple cloning site.
• The 6xHis tag on the expressed recombinant proteins allows for efficient coupling to Ni++ affinity resins and purification by a single step chromatography.
• As is the case with other protein tags, this polyhistidine tag can be cleaved at sites recognized by enzymes such as thrombin and enterokinases to isolate the protein of interest.
• Applications:Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry/Immunofluorescence.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 1:1000Immunoprecipitation: 1:25iImmunofluorescence (IC): 1:100.
• Requires Methanol permeabilization
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.