Glycogen synthase kinase 3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin.
GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase.
GSK-3 is a critical downstream element of the PI3 kinase/Akt cell survival pathway, and its activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3alpha and Ser9 of GSK-3beta.
GSK-3 has been implicated in the regulation of cell fate in Dictyostelium and is a component of the Wnt signaling pathway required for Drosophila, Xenopus and mammalian development.
GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization.
Applications:Suitable for use in Western Blot.
Other applications have not been tested.
Recommended Dilutions:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Specificity：Recognizes endogenous levels of human GSK-3beta only when phosphorylated at Ser9. May cross-react weakly with the phosphorylated form of GSK-3alpha due to high sequence homology. Species Crossreactivity: mouse, rat and monkey
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mk Mo Rt
Immunogen：Synthetic phosphopeptide derived from the sequence around Ser9 of human GSK-3beta. Species Sequence Homology: zebrafish and bovine
