Glutathione S-transferase is a 26kD protein from Schistosoma japonicum.
GST is a widely used protein fusion partner.
It provides both an easily detectable Tag.
Utilizing a simple purification process, there is little effect on the biological function of the protein of interest.
Numerous vectors containing GST-Tag have been developed for both prokaryotic and eukaryotic systems over the past decade.
Applications:Suitable for use in ELISA, Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Immunoprecipitation: 1:50Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Specificity：Detects overexpressed GST proteins and fusion proteins. Recognizes only glutathione S-transferase. Species Crossreactivity: Expected to crossreact with proteins fused to GST from all species.
Isotype：IgG
Calc Applications Abbrev：E IP WB
Calc Crossreactivity：Ma
Immunogen：GST fusion protein. Apparent Molecular Weight: The apparent molecular weight is related to the fusion protein.
