The Forkhead family of transcription factors is involved in tumorigenesis in rhabdomyosarcoma and acute leukemias.
Within the family, three members (Fox01, Fox03a and Fox01, also known as FKHR, AFX and FKHRL1) have sequence similarity to the nematode homologue DAF-16, which has been shown to mediate signaling via a pathway involving IGFR1, PI3K and Akt.
There are three Akt phosphorylation sites in the FKHR proteins: Thr24, Ser256 and Ser319.
Phosphorylation of FKHR family members at these sites by Akt promotes cell survival and regulates the cell cycle.
Phosphorylation regulates FKHR nuclear translocation and target gene transcription.
Forkhead transcription factors can also be inhibited by the deacetylase sirtuin (SirT1).
Applications:Suitable for use in ELISA, Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:1000.
Incubate membrane with diluted antibody in TBS, 5% BSA, 0.1% Tween-20 at 4°C with gentle shaking, overnight Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Specificity：Recognizes endogenous levels of human Fox01 when phosphorylated at Thr24 and Fox03a when phosphorylated at Thr32. Reacts with phosphorylated Fox04 at Thr28 but not with Fox01 family members phosphorylated at other sites. Species Crossreactivity: mouse, rat and monkey
Isotype：IgG
Calc Applications Abbrev：E IP WB
Calc Crossreactivity：Hu Mk Mo Rt
Immunogen：Synthetic phosphopeptide corresponding to residues surrounding Thr28 of human Fox04
