The Forkhead family of transcription factors is involved in tumorigenesis in rhabdomyosarcoma and acute leukemias.
Within the family, three members (FKHR, AFX and FKHRL1) have sequence similarity to the nematode homologue DAF-16, recently shown to mediate signaling via a pathway involving IGFR1, PI3K and Akt.
There are three Akt phosphorylation sites in the FKHR proteins: Thr24, Ser256 and Ser319.
Phosphorylation of FKHR family members at these sites by Akt promotes cell survival and regulates the cell cycle.
Phosphorylation regulates FKHR nuclear translocation and target gene transcription.
Applications:Suitable for use in Western Blot.
Other applications have not been tested.
Recommended Dilutions:Western Blot: 1:1000.
Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween 20 at 4°C with gentle shaking overnight
Optimal dilutions to be determined by the researcher.
Molecular Weight:~75kD
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography
Form：Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Specificity：Recognizes endogenous levels of total levels of human FKHR only when phosphorylated at Ser256. Crossreacts with phosphorylated AFX. Species Crossreactivity: mouse and rat.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic phosphopeptide corresponding to residues around Ser256 of human FKHR.
