Fas-associated death domain (FADD or Mort 1) functions as an important adaptor in coupling death signaling from membrane receptors, such as the Fas ligand and TNF family (DR3, DR4 and DR5), to caspase- 8 (1,2).
FADD has a C-terminal death domain interacting with the cytoplasmic tail of the membrane receptor and an N-terminal death effector domain interacting with caspase-8.
Clustering of the receptors upon stimulation brings FADD together further to oligomerize caspase-8 and activate the caspase signaling pathway.
Human FADD is phosphorylated mainly at Ser194, while mouse FADD is phosphorylated at Ser191.
In both cases, the phosphorylation is cell cycle dependent (3) and may be related to its regulatory role in embryonic development and the cell cycle process (4,5).
Applications:Suitable for use in ELISA and Western Blotting.
Other applications not tested.
Recommended Dilution:Western Blotting: 1:1000 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Specificity：Recognizes endogenous levels of human FADD protein.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to residues surrounding Ser194 of human FADD.
