The ErbB2 proto-oncogene encodes a transmembrane receptor-like glycoprotein of 185kD with intrinsic tyrosine kinase activity (1).
ErbB2 does not have any known ligand; however, the kinase activity of ErbB2 can be activated without any ligand if it is overexpressed and by heteroassociation with other members of the ErbB family (2).
Amplification of the ErbB2 gene and overexpression of its product are detected in almost 40% of human breast cancers (3).
Binding of c-Cbl ubiquitin ligase to Tyr1112 of ErbB2 leads to poly-ubiquitination of ErbB2 and enhances its degradation (4).
ErbB2 is one of the major targets for breast cancer and other carcinomas.
The therapeutic potential may result from directing ErbB2 to a c-Cblregulated proteolytic pathway.
Tyrosine 877 of ErbB2 is homologous to pp60c-Src Tyr416 located in the kinase domain.
Phosphorylation of this site may be involved in regulation of ErbB2 biological activity.
Tyrosine 1248 of ErbB2 is one of the major autophosphorylation sites in this molecule.
The phosphorylation of this site couples ErbB2 to the Ras-Raf-MAP kinase signal transduction pathway (5).
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Specificity：Recognizes human ErbB2 only when phosphorylated at Tyr1248. Exhibits crossreactivity with EGFR only when phosphorylated at Tyr1173.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic phosphopeptide corresponding to residues surrounding Tyr1248 of human ErbB2.
