84-1036-24　ErbB2 (c-erbB2HER2, neu Oncoprotein) 100ul　E3451-25A

特徴

• The ErbB2 proto-oncogene encodes a transmembrane receptor-like glycoprotein of 185kD with intrinsic tyrosine kinase activity (1).
• ErbB2 does not have any known ligand; however, the kinase activity of ErbB2 can be activated without any ligand if it is overexpressed and by heteroassociation with other members of the ErbB family (2).
• Amplification of the ErbB2 gene and overexpression of its product are detected in almost 40% of human breast cancers (3).
• Binding of c-Cbl ubiquitin ligase to Tyr1112 of ErbB2 leads to poly-ubiquitination of ErbB2 and enhances its degradation (4).
• ErbB2 is one of the major targets for breast cancer and other carcinomas.
• The therapeutic potential may result from directing ErbB2 to a c-Cblregulated proteolytic pathway.
• Tyrosine 877 of ErbB2 is homologous to pp60c-Src Tyr416 located in the kinase domain.
• Phosphorylation of this site may be involved in regulation of ErbB2 biological activity.
• Tyrosine 1248 of ErbB2 is one of the major autophosphorylation sites in this molecule.
• The phosphorylation of this site couples ErbB2 to the Ras-Raf-MAP kinase signal transduction pathway (5).
• Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000, incubate membrane with diluted antibody in TBS, 5% BSA, 0.1% Tween-20 at 4°C with gentle shaking, overnight
• Immunohistochemistry (Paraffin): 1:50Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.