DARPP-32 (dopamine and cyclic AMP-regulated phosphoprotein, relative molecular mass 32,000) is a cytosolic protein highly enriched in medium-sized spiny neurons of the neostriatum.
It is a bifunctional signaling molecule that controls serine/threonine kinase and serine/threonine phosphatase activity.
Dopamine stimulates phosphorylation of DARPP-32 through D1 receptors and activation of PKA.
PKA phosphorylation of DARPP-32 at Thr34 converts it into an inhibitor of protein phosphatase 1.
DARPP-32 is converted into an inhibitor of PKA when phosphorylated at Thr75 by cyclin-dependent kinase 5 (CDK5).
Mice containing a targeted deletion of the DARPP-32 gene exhibit an altered biochemical, electrophysiological and behavioral phenotype.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:1000.
Incubate membrane with diluted antibody in 1X TBS, 0.1% Tween-20, 5% BSA at 4°C with gentle shaking, overnight.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservatives added.
Specificity：Recognizes endogenous levels of total human DARPP32 at ~32kD, independent of phosphorylation. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to the residues surrounding Thr34 of human DARPP32.
