Death associated protein 5 (DAP5) is a 97kD protein with high amino acid sequence homology to eukaryotic translation initiation factor 4G (eIF4G) (1,2).
Compared with eIF4G, DAP5 lacks the N-terminal region necessary for cap-dependent translation and has a unique C-terminal part functioning as a regulator for interferongamma induced cell death (2,3).
During induced apoptosis, DAP5 is cleaved at Asp790.
The C-terminal truncated form of DAP5 functions as a cap-independent translation initiation factor responsible for the mediation of its own translation during apoptosis (4).
Applications:Suitable for use in ELISA and Western Blotting.
Other applications not tested.
Recommended Dilution:Western Blotting: 1:1000, incubate membrane with diluted antibody in 5% nonfat dry milk, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Specificity：Recognizes endogenous levels of total DAP5 protein independent of phosphorylation. Does not cross-react with other DAP family members or with eIF4G. Species Crossreactivity: Mouse and rat.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to residues surrounding amino acid 805 of human DAP5.
