c-Jun is a component of the transcription factor AP-1, which binds and activates transcription at TRE/AP-1 elements.
The transcriptional activity of c-Jun is regulated by phosphorylation at Ser63 and Ser73 (1,2).
Extracellular signals, including growth factors, transforming oncoproteins and UV irradiation, stimulate phosphorylation of c-Jun at Ser63/73 and activate c-Jun-dependent transcription.
Mutation of Ser63/73 renders c-Jun nonresponsive to mitogenic and stressinduced signaling pathways.
The MAP kinase homologue SAPK/JNK binds to the N-terminal region of c-Jun and phosphorylates c-Jun at Ser63/73.
In addition, the activity of SAPK/JNK is stimulated by the same signals that activate c-Jun (3,4).
Applications:Suitable for use in ELISA, Western Blot and Immunofluorescence.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Immunofluorescence (IF-IC): 1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Specificity：Detects endogenous levels of c-Jun only when activated by phosphorylation at Ser73. Also recognizes phosphorylation of JunD at Ser100. Species Crossreactivity: Human, mouse and rat.
Isotype：IgG
Calc Applications Abbrev：E IF WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic phosphopeptide corresponding to residues around Ser73 of human c-Jun.
