Entry of all eukaryotic cells into M-phase of the cell cycle is regulated by activation of cdc2 kinase.
Activation of cdc2 is controlled at several steps including cyclin binding and phosphorylation of threonine 161 (1–4).
However, the critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of tyrosine 15 and threonine 14 (3,5).
Phosphorylation at Tyr15 and inhibition of cdc2 is carried out by Wee1 and Myt1 protein kinases, while Tyr15 dephosphorylation and activation of cdc2 is carried out by the cdc25 phosphatase (3,4,6).
Applications:Suitable for use in Western Blot, Immunoprecipitation and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight
Immunoprecipitation: 1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Specificity：Recognizes endogenous levels of human cdc2, cdk2 and cdk5 only when catalytically inactivated by phosphorylation at Tyr15. Shows no crossreactivity with cdk4, cdk6 or cdk7. It does detect the yeast homologue of cdc2 (cdc28) when phosphorylated at Tyr19. Species Crossreactivity: Monkey, mouse, rat and Xenopus.
Isotype：IgG
Calc Applications Abbrev：E IP WB
Calc Crossreactivity：Hu Mk Mo Rt Xe
Immunogen：Synthetic phosphopeptide corresponding to residues around Tyr15 of human cdc2.
