CCAAT/enhancer-binding proteins (C/EBPs) are a family of transcription factors that are critical for cellular differentiation, terminal functions and inflammatory response (1).
Six members of the family have been characterized (C/EBP alpha, C/EBP beta, C/EBP delta, C/EBP epsilon, C/EBP gamma and C/EBP zeta) and are distributed in a variety of tissues (1).
Translation from alternative start codons results in three isoforms of C/EBP beta.
Two of them are transcriptional activators, also known as p35 and p32 LAPs (liver activating proteins).
The smaller isoform, p20 LIP (liver inhibitory protein), however, is a transcriptional inhibitor (2).
Phosphorylation of C/EBP beta at distinct sites stimulates its transcriptional activity (3–5).
Phosphorylation at serine 105 of rat C/EBP beta, a unique site only present in the rat sequence, seems essential for rat C/EBP beta activation (6).
Applications:Suitable for use in ELISA and Western Blotting.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Specificity：Recognizes endogenous levels of rat C/EBP• protein (phosphorylation-state independent). Does not cross-react with other C/EBP family members such as C/EBPalpha, -delta, -gamma, -epsilon or -zeta. It also does not recognize the p20 LIP rat C/EBP• isoform. Species Crossreactivity: Expected to crossreact with mouse according to sequence similarity.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Rt
Immunogen：Synthetic peptide corresponding to residues surrounding Ser105 of rat C/EBP beta.
