Beta-catenin is one of the key downstream effectors in the Wnt signaling pathway (1).
It has been implicated in two major biological processes of vertebrates: early embryonic development (2) and tumorigenesis (3).
GSK-3beta or other kinases may destabilize beta-catenin by phosphorylating it at serines 33, 37, 45 and/or threonine 41 (4).
Stabilizing mutations of these phosphorylation sites in beta-catenin have been found in many tumor cell lines (5).
Applications:Suitable for use in ELISA and Western Blot.
Other applications have not been tested.
Recommended Dilution:Western Blot: 1:1000 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Specificity：Recognizes endogenous levels of human beta-catenin only when phosphorylated at Thr41 or Ser45. This antibody does not recognize β-catenin phosphorylated at other sites.Species Crossreactivity: mouse and monkey
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mk Mo
Immunogen：Synthetic phosphopeptide corresponding to residues surrounding Thr41 and Ser45 of human β-catenin. Species sequence homology: canine and porcine
