Beta-catenin is a key downstream effector in the Wnt signaling pathway.
It is implicated in two major biological processes of vertebrates: early embryonic de- velopment and tumorigenesis.
CK1 phosphorylates b-catenin on Ser45.
This phosphorylation event primes b-catenin for subsequent phosphorylation by GSK-3.
GSK-3b destabilizes b-catenin by phosphorylating it at Ser33, 37 and Thr41.
Mutations in these phosphorylation sites, which result in the stabilization of b-catenin protein levels, have been found in many tumor cell lines.
Applications:Suitable for use in Western Blot..
Other applications have not been tested.
Recommended Dilutions:Western Blot: 1:1000 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Specificity：Recognizes endogenous levels of human beta-catenin only when phosphorylated at Ser33, Ser37 or Thr41. It does not recognize beta-catenin phosphorylated at other sites. Species Crossreactivity: rat, mouse and monkey.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mk Mo Rt
Immunogen：Synthetic phosphopeptide corresponding to residues surrounding Ser33, Ser37 and Thr41 of human beta-catenin. Species sequence homology: porcine, chicken, bovine, Xenopus, zebrafish and canine
