Beta-catenin is one of the key downstream effectors in the Wnt signaling pathway (1).
It has been implicated in two major biological processes of vertebrates: early embryonic development (2) and tumorigenesis (3).
GSK-3 beta or other kinases may destabilize beta-catenin by phosphorylating it at serines 33, 37, 45 and threonine 41 (4).
Stabilizing mutations of these phosphorylation sites in beta-catenin have been found in many tumor cell lines (5).
Applications:Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:1000Immunoprecipitation: 1:100 Immunohistochemistry (Paraffin): 1:400Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Specificity：Recognizes endogenous levels total of human beta-catenin. Does cross-react with endogenous levels of γ-catenin. Species Crossreactivity: mouse, monkey and rat.
Isotype：IgG
Calc Applications Abbrev：IHC IP WB
Calc Crossreactivity：Hu Mk Mo Rt
Immunogen：Synthetic peptide corresponding to residues around Ser37 of human beta-catenin. Species sequence homology: porcine and zebrafish
