Caspase-9 (ICE-LAP6, Mch6) is an important member of the cysteine aspartic acid protease (caspase) family.
Upon apoptotic stimulation, cytochrome c released from mitochondria associates with procaspase-9 (47kD)/Apaf 1.
This complex processes procaspase-9 into a large active subunit (35kD or 17kD) and a small subunit (10kD) by self-cleavage at (Asp315).
Cleaved caspase-9 further processes other caspase members, including caspase- 3 and caspase-7, to initiate a caspase cascade, leading to programmed cell death.
In addition to self-cleavage, procaspase-9 also can be cleaved in vivo by caspase-3 at Asp330.
This process serves as positive feedback to amplify the apoptotic signal in the caspase activation pathway.
Applications:Suitable for use in Western Blot.
Other applications have not been tested.
Recommended Dilution:Western Blot: 1:1000, incubate membrane with diluted primary antibody in 5% nonfat dry milk, 1X TBS, 0.1% Tween 20 at 4°C with gentle shaking, overnight
Optimal dilutions to be determined by researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Specificity：Recognizes endogenous levels of both full length mouse caspase-9 (49kD) and the large fragment of mouse caspase-9 following cleavage at Asp353 (37kD) and/or Asp368 (39kD). Does not react other caspases.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Mo
Immunogen：Synthetic peptide corresponding to residues surrounding the cleavage site of mouse caspase-9.
