Caspase-6 (Mch2) is one of the major executioner caspases functioning in cellular apoptotic processes (1,2).
Upon apoptotic stimulation, initiator caspases such as caspase-9 are cleaved and activated (3).
The activated upstream caspases further process downstream executioner caspases, such as caspase-3 and caspase-6, by cleaving them into large and small subunits, thereby initiating a caspase cascade leading to programmed cell death (4,5).
One of the major targets for caspase-6 is the membrane associated protein lamin A (6).
The cleavage of this protein causes cell membrane malfunction, membrane blebbing and eventual cell death.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000.
Detects a band at 18kD
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Specificity：Recognizes endogenous levels of the large subunit of active caspase-6 resulting from cleavage at Asp179 in human or Asp162 in mouse and rat (18kD). This antibody does not recognize other cleaved caspases. Species Crossreactivity: human and mouse
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to the C-terminal sequence of p18, the large subunit of rat caspase-6.
